Samples are procured from living patients at NHS sites in the UK which are regional specialist centres for Mesothelioma treatment. Samples procured from these centres are processed on site and stored using local HTA licensed storage facilities or in some cases, sent directly to researchers requiring fresh tissue.
Patient data is entered onto a web based Mesobank database by identified staff within the specialist centre. Batched processed samples from each site are transported by couriers to the central storage facility from where they can be dispatched to researchers. A Supply Agreement is in place between each donor site and the Royal Papworth Hospital NHS Foundation Trust (lead site), and an Individual Project Agreement is in place between the Royal Papworth Hospital NHS Foundation Trust and the central storage facility (Advent Bioservices Ltd).
Standard Operating Procedures are in place for sample procurement, processing and storage at each donor site and for the entry of clinical data onto the web-based database. Mesobank use third party laboratories for isolation of DNA and the creation of cell lines and ensure that Material Transfer Agreements are in place prior to samples being transferred to these centres of expertise. Specialist courier services are used to transport samples from donor sites to third party laboratories for processing or to the central storage facility.
Mesobank is fully compliant with the Human Tissue Act 2004. Codes of Practice are available to all staff and form the basis of SOPs regarding the acceptance of donated tissues to the tissue bank, documented consent, tracking, storage and disposal.
All fresh frozen tissue, pleural fluid and blood samples from living patients accepted into Mesobank have documented evidence of fully informed consent. Samples without this documentation will be subject to disposal if evidence of consent cannot be provided to the tissue bank. Mesobank also has Research Ethics Committee approval to collect pleural biopsy tissue taken from Mesobank donors, that is surplus to diagnostic need, and stored in local hospital archives in Formalin Fixed Paraffin Embedded (FFPE) blocks. These samples are not taken as part of the standard Mesobank procurement process.